TABLE 2.

Enzyme activities and short-term stress survival of original and new pcm mutants and complemented strains

Strain	Relevant genotype	Enzyme activitya		% Survival of stationary-phase cellsb
		Pcmc	HPII catalased	Heate	Saltf	15 mM H2O2g	30 mM H2O2g
MC1000	Parent strain	3.3 ± 0.61	36.0 ± 2.5	7.2	59	80	9.0
CL1010	Δpcm::KmrrpoS396	0.08 ± 0.04	3.1 ± 0.40	<1.0 × 10−5	3.0	34	<1.0 × 10−5
JV1012	rpoS13::Tn10	3.6 ± 0.25	0.63 ± 0.22	<2.2 × 10−5	2.4	1.0	<1.0 × 10−5
JV1026	Δpcm::KmrrpoS396 attB::pcm+	1.9 ± 0.25	3.9 ± 0.97	<2.5 × 10−5	2.1	39	5.0 × 10−5
HC1011	Δpcm::KmrrpoS396 attB::rpoS+	0.05 ± 0.03	30.2 ± 2.4	4.0	ND	80	4.0
JV1068	Δpcm::Cmr	0.0 ± 0.004	37.0 ± 1.3	5.2	54	82	10

^aAverages of at least four trials; error range represents 1 standard deviation. 

^bValues are averages of at least two trials in duplicate; ND, not determined. 

^cPcm activity in picomoles of methyl groups transferred to an isoaspartyl-containing peptide substrate per minute per milligram of total protein. 

^dHPII activity in micromoles of hydrogen peroxide decomposed per minute per milligram of total protein. 

^eDiluted 1:10 in 0.85% NaCl and heated to 55°C for 10 min. 

^fDiluted 1:10 in 2.5 M NaCl and incubated at 37°C for 4 h. 

^gDiluted 1:10 in H₂O₂ at indicated concentration; incubated at 37°C for 1 h.