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. 2023 Dec 14;12:RP85562. doi: 10.7554/eLife.85562

Figure 2. The expression of several actors of steroidogenesis is downregulated in 30 day organotypic cultures.

Figure 2.

(A–I) Relative mRNA levels of Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b3, and Hsd17b2 (normalized to Gapdh and Actb or to Hsd3b1) and relative protein levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) (normalized to ACTB) and CYP17A1 (normalized to 3β-HSD) during mouse postnatal development (6 dpp, 22 dpp, and 36 dpp) and in in vitro cultured fresh testicular tissues (FT) or controlled slow freezing (CSF) tissues (D16 and D30). (J) Representative images of CYP17A1 expression during mouse postnatal development (22 dpp and 36 dpp) and in in vitro cultured tissues (D16 and D30). A representative image of a negative control, carried out by omitting the primary antibody, is also shown. Testicular tissue sections were counterstained with Hoechst (blue). Dotted lines delineate seminiferous tubules. Scale: 15 µm. Data are presented as means ± SEM with n=4 biological replicates for each group. A value of *p<0.05 and **p<0.01 were considered statistically significant.

Figure 2—source data 1. Source data of Figure 2.