Skip to main content
. 2023 Dec 14;12:RP85562. doi: 10.7554/eLife.85562

Figure 3. An increased production of progesterone and estradiol and a decreased production of androstenedione are observed after 16 and 30 days of culture of prepubertal mouse testicular tissues.

Intratesticular concentrations of (A) progesterone, (B) androstenedione, (C) testosterone, and (D) estradiol during mouse postnatal development (6 dpp, 22 dpp, and 36 dpp) and in in vitro cultured fresh (FT) or frozen/thawed (CSF) tissues (D16 and D30). Steroid concentrations were normalized to protein levels. (E–F) Concentrations of (E) progesterone, androstenedione, testosterone, and (F) estradiol in the culture medium of FT tissues. (G) Intratesticular concentrations of total, free, and esterified cholesterol normalized to tissue mass. Data are presented as means ± SEM with n=4 biological replicates for each group. A value of *p<0.05 was considered statistically significant.

Figure 3—source data 1. Source data of Figure 3.

Figure 3.

Figure 3—figure supplement 1. Impact of controlled slow freezing on steroids production by Leydig cells in organotypic cultures.

Figure 3—figure supplement 1.

Concentrations of androgens (androstenedione and testosterone) in the culture medium of fresh (FT) or frozen/thawed (CSF) testicular tissues. Data are presented as means ± SEM with n=4 biological replicates for each group. A value of *p<0.05 and ***p<0.001 were considered statistically significant.
Figure 3—figure supplement 1—source data 1. Source data of Figure 3—figure supplement 1.