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. 2023 Nov 8;8(21):e167049. doi: 10.1172/jci.insight.167049

Figure 5. TGF-βR1 is a key downstream mediator of Sema3A/NRP1 signaling.

Figure 5

(A and B) Representative immunostaining images and quantitation of EdU-positive cells in NT (control) and TGF-βRI–KD GBM cells treated with rSema3A. TGF-βRI KD was confirmed by IB analysis. Scale bar: 50 μm. n = 5. (C) LDA of 131 GBM cells treated with a TGF-βR inhibitor, SB431542 (2 μM), and rSema3A. n = 5. (D) Kaplan-Meier survival curves of mice orthotopically implanted with 559 GBM cells transduced with NT shRNA (control, n = 6), NRP1 shRNA (n = 7), NT shRNA + T204D TGF-βR1 mutant (n = 7), and NRP1 shRNA + T204D TGF-βR1 mutant (n = 8). *P < 0.001 by log-rank test. Data represent mean ± SD. *P < 0.01 by 1-way ANOVA with Tukey’s multiple-comparison test in B and C.