TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| Klebsiella oxytoca derivatives | ||
| M5a1 | Wild type | 1 |
| CYME | Like M5a1, Δ(cymE) | This work |
| Escherichia coli K-12 derivatives | ||
| JM109 | F′ (traD36 proAB+ lacIqZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17 relA1 supE44 Δ(lac-proAB) λ− | 46 |
| MC4100 | F−, Δ(argF-lac) U169 araD139 ptsF25 deoC1 relA1 flbB5301 rpsL150 λ− | 13 |
| GM1 | Like MC4100, malE::Tn10 (Kanr) | 17 |
| JV2000 | Like MC4100, malS::Tn10 (Tetr) phoA8 rbsR | W. Boos |
| GM15 | Like GM1, malS::Tn10 (Tetr) | This work |
| Escherichia coli B derivatives; BL21 (DE-3) | F−hsdS gal λ(DE-3) | 41 |
| Plasmids | ||
| pT7-H6-TRXFUS | AprH6-trxA | 22 |
| pCYME2 (pT7-H6-TRXFUS derivative) | AprH6-trxA ′cymE | This work |
| pSU2719 | CmrlacZα | 25 |
| pGM200 (pSU2719 derivative) | CmrcymA-J | 17 |
| pCYMΔE (pSU2719 derivative) | CmrcymA-D Δ(cymE) cymF-J | 17 |