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. 2023 Nov 22;8(22):e171054. doi: 10.1172/jci.insight.171054

Figure 1. hPF4 and KKO inhibit fibrin generation initiated by LMW NETs and DNA.

Figure 1

Fibrin generation in pooled normal plasma (PNP) with or without added HMW or LMW NETs (blue) or DNA (purple). DN1, DNase I; Af2, AflII; BG1, BsrGI-HF; Al1, AluI. Data are mean ± SEM of at least 3 independent experiments. (A) Lag time determined from kinetic curves of fibrin generation with NETs (blue) and DNA (purple). (B) Slope (rate) of fibrin generation was determined from the same kinetic curves as in A. (C) Lag time of DNA-induced fibrin generation was determined from kinetic curves similarly to A, in the presence of hPF4 (20 μg/mL). (D and E) Lag times are shown for fibrin generation studies of DNase I–digested LMW NETs (D) and DNA (E) in the absence or presence of low hPF4 (1 μg/mL) and/or KKO (10 μg/mL). Data are mean ± SEM of at least 3 independent experiments. Comparative statistical analysis was performed by Kruskal-Wallis 1-way ANOVA.