Figure 3. Effect of hPF4 on fibrin generation in FXI- and FXII-depleted plasma and on thrombogenicity of ssDNA.

(A) Lag time, determined from kinetic curves, of fibrin generation in PNP or FXI- or FXII-depleted (dep) plasma, induced by DNase I–digested LMW DNA in the absence or presence of hPF4. (B) Fibrin generation lag time in FXI-depleted plasma spiked with CTI, induced by LMW DNA in the absence or presence of hPF4. (C) Lag time of fibrin generation induced by LMW DNA in depleted plasma supplemented with missing coagulation factors. (D) Lag time of ssDNA-induced (pink) fibrin generation compared to dsDNA (purple). (E) Lag time of ssDNA-induced fibrin generation with added hPF4 (20 μg/mL). Data are mean ± SEM of at least 3 independent experiments. Comparative statistical analysis was performed by Kruskal-Wallis 1-way ANOVA.