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. 2023 Nov 22;8(22):e171962. doi: 10.1172/jci.insight.171962

Figure 4. Histological and histomorphometric analysis of the distal part of the femur for osteoblasts and osteocytes in Sham-operated and OVX mice with or without intraperitoneal injection of 6A-8R (4 mg) 5 days per week for 4 weeks.

Figure 4

(A) Osteocalcin staining. (B) Sclerostin staining. Scale bars (A and B): 1 mm (top rows) and 200 μm (bottom rows). (C) Plot of the number of osteocalcin-positive cells per unit trabecular surface. (D) Plot of the number of sclerostin-positive cells per total osteocytes. Data are expressed as mean ± SD (n = 7 or 9). (E) Plots of the number of osteoblasts (Ob.N) (N/mm), bone formation rate (BFR)/bone surface (BS) (mm³/mm²/year), and mineral apposition rate (MAR) (μm/day). (F) Images of MAR under fluorescent light (white arrow, double-labeled surface). Scale bars: 10 μm. Data are expressed as mean ± SD (n = 4) and were statistically analyzed using 1-way ANOVA and the Tukey-Kramer test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.