Fig. 2. Cytotoxic effect of Ad5/3-E2F-d24-vIL2 plus adoptive NK cell therapy in human ovarian cancer tumor digests.

A–L Individual real time responses of ovarian cancer samples treated with vIL-2 virus in combination with allogeneic adoptive cell therapy. Ovarian cancer human samples were seeded in duplicates at the concentration of 5 × 10⁴ cells per well into pre-coated impedance plates with 5 µg/ml of human fibronectin. Cells were incubated at 37 °C for 24 h, when freshly isolated and expanded allogeneic NK cells were added in 1:8 effector to target ratio (E:T) to the plates. Concurrently, Ad5/3-E2F-d24-vIL2 or Ad5/3-E2F-d24 virus treatments (100 vp/cell) were added to the co-cultures. Untreated samples digests were used as a mock and NK cell monotherapy as a control groups for the assay. Cytotoxicity effect in co-cultures was evaluated through plate scans every 15 min for a total of 190 h. Normalized cell index to each cell index time-point was used as the final readout for the assay. Data sets were analysed for statistical significance by unpaired T test and presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p 0.001.