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. 2023 Dec 14;14(12):830. doi: 10.1038/s41419-023-06358-y

Fig. 6. USP44 inhibits Hh signaling-dependent growth and metastasis of HCC in vivo.

Fig. 6

A Nude mice were subcutaneously injected with HCCLM3 cells stably expressing indicated plasmids. Intratumor protein levels of Itch and Gli1 in indicated groups were detected by western blot. B BALB/C nude mice subcutaneously injected with luciferase-expressing HCCLM3/Vector+shCtrl, HCCLM3/USP44+shCtrl, HCCLM3/Vector+shItch, or HCCLM3/USP44+shItch cells were assessed by IVIS imaging system. C, D Volume and weight of tumors in indicated groups (n = 5 per group). E IVIS imaging system was used to detect the effect of USP44 on metastasis of HCC in nude mice with orthotopic implanted xenografts. F Quantification of lung metastasis in the indicated groups of mice (n = 5 per group). G Nude mice were subcutaneously injected with HCCLM3 cells stably expressing indicated plasmids. Intratumor protein levels of Itch and Gli1 in indicated groups were detected by western blot. H Nude mice subcutaneously injected with luciferase-expressing HCCLM3/Vector, HCCLM3/USP44, HCCLM3/USP44+Gli1 WT, or HCCLM3/USP44+Gli1 MUT cells were assessed by IVIS imaging system. WT, wild-type; MUT, mutant. I, J Volume and weight of tumors in HCCLM3/Vector, HCCLM3/USP44, HCCLM3/USP44+Gli1 WT, or HCCLM3/USP44+Gli1 MUT groups. K IVIS imaging system was used to detect the effect of USP44 on metastasis of HCC in nude mice with orthotopic implanted xenografts. L Quantification of lung metastasis in the indicated groups of mice (n = 5 per group). Data are obtained from three independent biological replicates and are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 as indicated.