TABLE 4.
Stress and analog induction of dctAp
| Strain | Genotype | β-Galactosidase activity
under the following growth conditiona:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Glc-10 mM NH4 | Glc-0.5 mM NH4 | Glc-NH450 mM sucrose | Glc-NH4100 mM sucrose | Glc-NH4itaconic acid | Glc–NH4– Me-Succ | Glc–NH4– DiMe-Succ | Glc-Asn | Glc-AsnNH4 | ||
| 3841 | Wild type | 266 ± 61 | 1,040 ± 230 | 231 ± 38 | 236 ± 41 | 1,317 ± 118 | 199 ± 7 | 202 ± 3 | 290 ± 5 | 229 ± 41 |
| RU727 | dctA::Ω | 297 ± 25 | 1,362 ± 81 | 406 ± 57 | 2,011 ± 322 | 1,889 ± 233 | 1,777 ± 103 | 1,530 ± 214 | 782 ± 88 | 505 ± 71 |
| RU730 | dctB::Ω | 184 ± 27 | 182 ± 43 | 266 ± 14 | 210 ± 8 | 75 ± 21 | 143 ± 27 | 65 ± 28 | 87 ± 63 | |
| RU711 | dctD::Ω | 150 ± 26 | 252 ± 62 | 191 ± 14 | 171 ± 25 | 211 ± 81 | 164 ± 37 | 85 ± 25 | 94 ± 5 | |
| RU865 | ΔdctBD::Ω | 168 ± 10 | 204 ± 44 | 117 ± 12 | 78 ± 21 | |||||
| RU714 | ΔdctABD::Ω | 179 ± 65 | 205 ± 35 | 123 ± 41 | 168 ± 51 | |||||
| RU929 | ntrC::Ω | 139 ± 10 | 93 ± 15 | |||||||
a
Results are shown as o-nitrophenylgalactoside hydrolyzed (in nanomoles per minute per milligram of protein ± SEM) and are based on at least three independent cultures assayed in triplicate. In all cases, the lacZ fusion is carried by pRU103. Unless otherwise stated, all compounds are at 10 mM concentrations. Glc, glucose; Me-Succ, 2-methyl succinate; DiMe-Succ, 2,2-dimethyl succinate.