Figure 2:

Bispecific AAVs preferentially transduce CD4+/CD32a+ cells in the presence of CD32a+ and CD4+ cells.

Gene transfer activities mediated by the indicated four AAV vectors (GC/cell of 2.67×10⁴) into SupT1-CD4/CD32a cells were determined in the presence of SupT1-CD4 and SupT1-CD32a cells three days post-transduction. A) GFP expression was determined in a 1:1:1 mixture of SupT1-CD4, SupT1-CD32a and SupT1-CD4/CD32a cells. Flow cytometry plots provide the percentages of CD4+ and CD32a+ cells among all GFP+ cells. B) Percentage of GFP expression in SupT1-CD4/CD32a cells serially diluted into a 1:1 co-culture of SupT1-CD4 and SupT1-CD32a cells. C) Ratios of CD4+CD32a+GFP+ cells over CD32a+GFP+ cells. D) Ratios of CD4+CD32a+GFP+ cells over CD4+GFP+ cells. B-D) Experiments were performed in three independent replicates, and statistical differences were calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. The standard deviation is reported. P-value **** < 0.0001, p-value *** = 0.0001–0.001, p-value ** = 0.001–0.01, p-value * = 0.01–0.05. Only significant and relevant statistical comparisons are shown. The statistical comparison of all groups in each data set is available in the supplements.