Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 14;14:7918. doi: 10.1038/s41467-023-43587-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Scheme for the iPGC induction in vivo using germplasm. b At the 1-cell stage, zebrafish embryos were injected with buc (200 pg), 13GM (50 pg per fraction), or 9GM (50 pg per fraction) mRNA to induce iPGCs. GFP-UTRnanos3 was used to label putative PGCs. The embryos were photographed at 90% epiboly stage. c Schematic diagram for iPGCT. d Time-lapse of iPGC migration to the genital ridges after iPGCT. GFP-UTRnanos3 was used to label iPGCs. e GFP-UTRnanos3 was used to label PGCs and iPGCs, except that immunofluorescence against Piwil1 was used to visualize endogenous PGCs of wild-type embryos at 30% epiboly. f Number of PGCs in wild-type and iPGCT embryos at different stages. Each point represents an independent sample (n ≥ 6). g iPGCs migrated to the genital ridge of the host embryo. h Success rates of iPGCT and conventional PGCT at 1 dpf and 4 dpf. Each point represents an independent experiment (n ≥ 3). i Immunofluorescence detection of Vasa protein in GFP-UTRnanos3-positive iPGCs of iPGCT embryos at 8 dpf. j Probes were designed at the UTR of mRNA (en_probes) to distinguish neonatal mRNA, and single molecule in situ hybridization was used to detect the neonatal germplasm mRNA of ddx4 and tdrd7a in the GFP-UTRnanos3-positive iPGCs and ePGCs at 1 dpf. A representative example of three replicate is shown. All data are presented as mean values ± SEM. Two-tailed Student’s t-test was used to calculate the P values.