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. 2023 Dec 14;14:7918. doi: 10.1038/s41467-023-43587-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic diagram for iPGCT using Tg(cmv:GFP) embryos as iPGCT donors and Tg(cmv:mCherry) embryos as iPGCT hosts. b After iPGC transplantation, host embryos expressed mCherry and germ cells expressed GFP. c Immunofluorescence detection of Vasa in GFP-positive germ cells of iPGCT gonads at 32 dpf. Note that gonadal somatic cells expressed mCherry. d H&E staining of Tg(cmv:GFP) and iPGCT testes. SC spermatocyte, ST spermatid, SZ spermatozoa. e Immunofluorescence detection of Sycp3 and PCNA showing normal meiosis and mitosis of germ cells in iPGCT testis and control testis at 90 dpf. f Immunofluorescence detection of Vasa in GFP-positive germ cells of iPGCT testes at 90 dpf. Rectangular boxes 1 and 2 show the development of gonads on both sides of the iPGCT embryo, respectively. g The iPGCT fish expressing mCherry produced sperm expressing GFP, and F1 and F2 generations expressing GFP were obtained. A representative example of three replicate is shown.