Table 2.
Proportion of cells in different positions of the embryo induced to PGC
| Injection location | Sample | Location of positive cells | ||
|---|---|---|---|---|
| Genital ridge (%) |
Trunk (%) |
Brain (%) |
||
| Animal pole | – | 0 | 0 | 100 (n = 44) |
| +buc | 0 | 0 | 100 (n = 38) | |
| +9GM | 100 (n = 48) | 0 | 10.42 (n = 5)a | |
| Margin | – | 0 | 100 (n = 58) | 0 |
| +buc | 0 | 100 (n = 66) | 0 | |
| +9GM | 100 (n = 62) | 12.9 (n = 11)a | 0 | |
Different combinations of mRNA were injected at the animal pole of 128-cell stage embryos. mCherryCAAX was used to label injected cells, GFP-UTRnanos3 was used to label iPGCs, and buc or 9 germplasm (9GM) was used to induce iPGCs.
aA small number of positive cells migrated to the head or trunk, and most of the positive cells migrated to the genital ridge.