Table 3.
Positive rate of offspring with different strategies
| Gene | Method | PGC-positive embryos/manipulated embryos (4 dpf) | Fluorescence positive embryos/injected embryos | Caudal fin positive adults (KI)/tested adults | Fertile adults (KI + iPGCT)/adults originated from PGC-positive embryos at 4 dpf | F0 adults producing positive F1/tested adults (%) | F0 adults producing positive F1/manipulated embryos (%) |
|---|---|---|---|---|---|---|---|
| sox19b | KI | – | 39/403 | 4/39 | – |
2/39 (5.13%) |
2/403 (0.50%) |
|
KI +iPGCT |
20/35 | – | – | 8/19 |
4/19 (21.05%) |
4/35 (11.43%) |
|
| mpx | KI | – | – | 14/384 | – |
8/384 (2.08%) |
8/384 (2.08%) |
|
KI +iPGCT |
15/23 | – | – | 7/12 |
7/12 (58.33%) |
7/23 (30.43%) |
|
| nanog | KI | – | 37/339 | 4/37 | – |
1/37 (2.70%) |
1/339 (0.29%) |
|
KI +iPGCT |
20/32 | – | – | 15/17 |
1/17 (5.88%) |
1/32 (3.13%) |