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. 2023 Dec 14;14:8341. doi: 10.1038/s41467-023-44089-y

Fig. 4. Cryo-EM structure of the ternary complex of TOP2B-etoposide-EGR1 TSS.

Fig. 4

a Density maps of the ternary complex in three different angles. Two TOP2B proteins shown in green and cyan, EGR1 TSS in yellow. b The ternary structure in ribbons. The DNA cleavage and etoposide binding sites, marked in a black box, are shown in close-up views. Two etoposide molecules are shown in sphere models, with the carbon and oxygen atoms colored white and red, respectively. The approximate angle of DNA bending is shown. c Schematic representation of the entire TOP2B protein. d qRT-PCR results indicating transcriptional activation and repression at the EGR1 and FOS genes in the HEK293 cells treated with etoposide and ICRF193, respectively (n = 3 biologically independent samples). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. Source data are provided as a Source Data file.