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| Number of cell lines surviving to passage |
|||||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| FBS | 9 | 5 | 3 | 2 | 2 | 1 | 1 |
| KnockOut SR | 47 | 26 | 7 | 2 | 1 | 1 | 0 |
In total, we cultured 1.2 × 105 and 8.8 × 105 cat embryonic fibroblasts in the medium supplemented with fetal bovine serum (FBS) and KnockOut serum replacement (KnockOut SR), respectively. Primary colonies were selected and cultured in the same medium.
