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. 2023 Sep 30;69(6):308–316. doi: 10.1262/jrd.2023-023

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Fig. 1. — Pituispheres of CD9/SOX2-positive cells in the intermediate lobes (IL) side of the marginal cell layer (MCL). (A) Double immunofluorescence staining for CD9 (red) and SOX2 (green) using CD9-positive pituispheres. The left panel shows a bright field (BF) image of the pituisphere. The right panel shows the merged image with DAPI (blue). (B) Hormone-producing cells in the CD9-positive pituispheres after induction. BF images and merged images with DAPI (blue), CD9 (red), and GH (green), or TSH (green) are shown. Black and white arrowheads indicate GH or TSH single-positive cells and CD9/GH or CD9/TSH double-positive cells, respectively. Scale bars: 20 μm (A and B).