TABLE 2.
Expression levels of isoenzymes in the various strainsa
| Strain or culture | mRNA expression levels
|
|||||
|---|---|---|---|---|---|---|
| F420-MDH | H2-MDH | Ratio | MCR I | MCR II | Ratio | |
| Wild type | 1 (1) | 1 (2) | 0.5 | 1 (1) | 1 (2) | 0.5 |
| Enrichment culture | 0.5 | 0.3 | 0.9 | NDb | 0.1 | |
| JB1 | 0.25 | 0.25 | 0.5 | 0.5 | 0.25 | 1 |
| JB2 | 0.15 | 0.15 | 0.5 | ND | 0.15 | |
| JB3 | 0.1 | 0.1 | 0.5 | 0.3 | 0.1 | 1.5 |
| JB4 | 0.3 | 1 | 0.15 | 0.6 | 1 | 0.3 |
| JB5 | 0.17 | 0.03 | 3 | 0.1 | 0.1 | 0.5 |
| JA1 | 0 | 0.06 | 0 | 0 | 0.06 | 0 |
a
The amounts of mRNA were estimated by dot blot analyses, with that of the wild-type strain taken as 1. Culturing and other methods were performed as described in the text. Values in parentheses refer to the ratio between wild-type mRNA levels of both forms of MDH and MCR, respectively.
b
ND, not detectable.