Figure 4.

Circulating T CD4⁺, T CD8⁺ and B CD19⁺ cells in peripheral blood of immunosuppressed mice. T CD4⁺ (A), T CD8⁺ (B) and B CD19⁺ (C) cells were analyzed by flow cytometry. Results are shown as the mean ± standard deviation for each group at W1, W6 and W9. The dotted lines show the normal values for each population: T CD4 + 2059.38 ± 720.18/mm³; T CD8 + 247.11 ± 70.94/mm³ and B CD19 + 51.13 ± 30.70/mm³ determined in-house in naïve mice of the same age. Significant differences within groups at W6 and W9 are marked with a * sign; a ⁺ sign indicates significant differences compared to the control group for the same week; a ^x sign indicates differences between immunosuppressed groups at W6 or W9 (p<0.05) **p<0.01; ***p<0.001; ⁺⁺p<0.01; ⁺⁺⁺⁺p<0.0001.