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. 2023 Dec 1;14:1285943. doi: 10.3389/fimmu.2023.1285943

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Copyright © 2023 Bernardo, Solana, Sánchez, Torres, Reyes-Cruz, Carrillo and Moreno

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-10 and PD-1 expression by B CD19⁺, T CD4⁺ and T CD8⁺ cells. After 72 h of SLA stimulation, splenocytes were stained and analyzed by flow cytometry to determine the frequency of B CD19⁺ cells producing IL-10 (A), as well as the expression of the cell exhaustion marker PD-1 by B CD19⁺ cells (B), T CD4⁺ cells (C) and T CD8⁺ cells (D). The results are expressed as the SLA/RPMI ratio, and shown as median and whisker plots. Significant differences within groups at W6 and W9 are marked with a * sign; a ⁺ sign indicates significant differences compared to the control group for the same week; a ^x sign indicates differences between immunosuppressed groups at W6 or W9 (p<0.05) **p<0.01; ***p<0.001; ⁺⁺p<0.01.