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. 2023 Dec 1;14:1285943. doi: 10.3389/fimmu.2023.1285943

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Copyright © 2023 Bernardo, Solana, Sánchez, Torres, Reyes-Cruz, Carrillo and Moreno

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total percentage of T-producing lymphocytes in the spleen of immunosuppressed mice, following SLA stimulation. Immunosuppressed and infected BALB/c mice were euthanized before (W6) and after (W9) Glucantime^® treatment. The splenocytes were stimulated for 72 h with L. infantum SLA to determine, by flow cytometry, the cytokine-producing capacity of the T CD4⁺ and T CD8⁺ cells via a Boolean gating analysis. Results are shown as the total percentage of single- (+), double- (++) and triple- (+++) cytokine-producing CD4⁺ T (A) and CD8⁺ T (B) among T lymphocytes, before and after antimonial treatment.