TABLE 1.
Bacterial strains and plasmids used in these studies
| Bacterial strain or plasmid | Genotype and/or relevant phenotypea | Reference or source |
|---|---|---|
| Bacteria | ||
| B. subtilis | ||
| PS356 | ΔsspA ΔsspB trpC2 α− β− | 14 |
| PS832 | trp+ revertant of strain 168 (wild type) | Laboratory stock |
| PS2437 | ΔsspA ΔsspB ΔsspE trpC2 pSspCwt α− β− γ− Cmr Kmr; expresses high levels of SspC in spores | 9 |
| PS2438 | ΔsspA ΔsspB ΔsspE trpC2 pSspCG52A α− β− γ− Cmr Kmr; expresses high levels of SspCG52A in spores | 9 |
| PS2638 | msrA::pPS2519 CmrmsrA | This study |
| PS2639 | msrA::pPS2519 ΔsspA ΔsspB α− β− CmrmsrA | This study |
| E. coli | ||
| PS708 | pPS708 Ampr; expresses SspC under control of the lac promoter | 24 |
| PS837 | pPS837 Ampr; expresses SASP-C under control of the lac promoter | 24 |
| Plasmids | ||
| pJH101 | Ampr Cmr Tetr | 7 |
| pPS708 | pDG148 with a 0.6-kb fragment containing the gene encoding SspC under control of the lac promoter | 24 |
| pPS837 | pDG148 with a 0.7-kb fragment containing the gene encoding SASP-C under control of the lac promoter | 24 |
| pSspC | pUB110 with sspC under the control of the strong forespore-specific sspB promoter | 37 |
| pSspCG52A | pUB110 with the gene encoding SspCG52A under the control of the sspB promoter | 37 |
| pPS2519 | pJH101 with a 0.25-kb fragment of msrA | This study |
a
Abbreviations: Ampr, ampicillin (50 μg/ml) resistance; Cmr, chloramphenicol (3 μg/ml) resistance, Kmr, kanamycin (10 μg/ml) resistance; Tetr, tetracycline (10 μg/ml) resistance; α−, β−, and γ−, lacking SASP-α, β, or γ, respectively.