TABLE 1.
Specifications for synthesised DNA oligonucleotides as proposed by the applicant (Documentation provided to EFSA No. 1).
| Parameter | Specification |
|---|---|
| Definition | Synthetic deoxyribonucleic acid |
| Colour Index No | Colourless |
| Chemical names | Deoxyribonucleic Acid (DNA) |
| Chemical formula | ■■■■■ |
| Molecular weight | ■■■■■ |
| Particle size of powder | ■■■■■ |
| Assay | Absorbance wave 260/280 OD reading |
| Spectrophotometry, spectrometry, chromatography, Infra Red, X‐ray diffraction | Spectrophotometry |
| Density/specific gravity | 1.7 g/cm3 |
| pH | Buffer dependent, Neutral pH |
| Precipitation reaction | Precipitation by ethanol when suspended in solution |
| Colour reaction | Sybr green chemistry is used for detection of the DNA |
| Solubility | 100% in Water |
| Specific identification tests and parameters | Quantitative PCR (qPCR) is used to detect, characterise and quantify the DNA using non specific Sybr Green Chemistry |