Table 1. Chromatographic and Spectrometric Parameters of the Analytes 1–3 and M1–M3.

analyte	RT (min)a	Q1/Q3 (m/z)b	calibrated range (R2)c	LLOQ (nM)d	precision (%)e	accuracy (%)e
1	5.02 (±0.01)	319.1/275.2*, 273.2	15.6 – 500.0 (0.993)	15.6 (S/N ≥ 31)	2.7–17.7	96.3–104.7
2	4.38 (±0.01)	481.2/118.9, 59.1*	31.3 – 500.0 (0.990)	31.3 (S/N ≥ 36)	4.4–24.3	86.1–107.8
3	5.21 (±0.01)	727.3/643.3*, 625.4	2.0 – 500.0 (0.998)	2.0 (S/N ≥ 23)	1.0–18.9	96.4–104.2
M1	4.38 (±0.01)	495.2/319.2*, 192.9, 174.8	2.0 – 250.0 (0.997)	2.0 (S/N ≥ 39)	2.0–16.1	83.7–109.5
M2	4.88 (±0.01)	495.2/319.2*, 174.9, 113.0, 84.9	0.5 – 62.5 (0.996)	0.5 (S/N ≥ 18)	2.6–24.7	85.2–106.5
M3	5.09 (±0.01)	495.2/319.1, 192.9*, 113.0, 59.0	1.0 – 62.5 (0.994)	1.0 (S/N ≥ 15)	2.3–18.1	88.6–108.7
4 (IS)	5.46 (±0.01)	315.1/271.2, 253.2*

^aCalculated from plasma analyses and plasma calibration standards.

^bQuantifier is marked w/asterisk.

^cCalibrated range with precision <25% at LLOQ and accuracy 80–120%.

^dLLOQ: lower limit of quantitation defined as the smallest concentration in the calibrated range.

^eValues from back-calculated calibration standards in the plasma matrix (cf. Supporting Information).