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Specific ability to prevent aggregation of the 58-kDa protein. An aliquot (15 μl) of the acid-unfolded DMSOR in refolding buffer was mixed with an equal volume of the purified protein (A), GroEL (B), or BSA (C) at final concentrations of 2.5, 5, 10, 25, and 50 μg per ml in 30-μl reaction mixtures. N, native DMSOR; U, acid-unfolded DMSOR with no sample.
