Figure 5.

Turbidimetric studies of gelatin nanoparticle formation. (a) Ultraviolet–visible spectra of an aqueous gelatin solution (M_w = 179 kDa; type B) as a function of ethanol addition (the inset shows the turbidity of gelatin solutions upon gradual addition of ethanol; the red dotted circle corresponds to the initial turbidity change). (b) Turbidity of the gelatin solution (M_w = 179 kDa; type B) at a fixed wavelength of 600 nm as a function of ethanol volume fraction. (c) Turbidity of the gelatin solution (M_w = 276 kDa; type A) at a fixed wavelength of 600 nm as a function of ethanol volume fraction. Ethanol volume fractions corresponding to the onset (end of phase I) and end of the rapid turbidity increase (end of phase II) are denoted as ϕ_t0 and ϕ_tm, respectively.