TABLE 1.
R. solanacearum strains and plasmids
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| Strain | ||
| AW | Wild-type pathogen; LacZ− | 29 |
| AW201 | epsA1::nptI EPS− LacZ− Kmr | 19 |
| AW-D5 | vsrD5::TnphoA LacZ− Kmr | This study |
| AW1-80 | phcA80::Tn5 LacZ− Kmr | 1 |
| Plasmid | ||
| pCB5 | pLAFR3 cosmid with eps and rgnII; Tcr | 6 |
| pTZRLZ7 | 2.7-kb BamHI fragment of pCB5 on pTZ18U; Apr | This study |
| pTZRLZ3 | 610-bp BamHI fragment of pJH161 on pTZ18U; Apr | This study |
| pTZRLZ1 | BamHI fragment from pRLZ1 on pTZ18U; Apr | This study |
| pRG970 | Transcriptional fusion vector with promoterless lacZ; Spr | 39 |
| pRLZ7 | pRG970 with nt −2400 to +275 of PxpsR fused to lacZ; Spr | This study |
| pRLZ1 | Same as for pRLZ7 except nt −338 to +36 | This study |
| pRLZ8 | Same as for pRLZ7 except nt −310 to +36 | This study |
| pRLZ4 | Same as for pRLZ7 except nt −286 to +36 | This study |
| pRLZ5 | Same as for pRLZ7 except nt −236 to +36 | This study |
| pRLZ2 | Same as for pRLZ7 except nt −117 to +36 | This study |
| pRLZ6 | Same as for pRLZ7 except nt −76 to +36 | This study |
| pRLZ15 | Same as for pRLZ1 except nt −322 CCC→TGG | This study |
| pRLZ16 | Same as for pRLZ1 except nt −319 to −316 (CTAA) deleted | This study |
| pRLZ17 | Same as for pRLZ1 except nt −327 AAT→GTA | This study |
| pRMB-S | Same as for pRLZ2 except nt −82 TAAAAA→AGATCT | This study |
| pRM5-S | Same as for pRLZ2 except nt −73 T→C | This study |
| pRM7-S | Same as for pRLZ2 except nt −83 T→C | This study |
| pRMB | Same as for pRLZ1 except nt −82 TAAAAA→AGATCT | This study |
| pRM7 | Same as for pRLZ1 except nt −83 T→C | This study |
| pRMB-M | Same as for pRMB except nt −286 to +36 | This study |
| pRM7-M | Same as for pRM7 except nt −286 to +36 | This study |
| pRLZ11 | Same as for pRLZ1 except has 4-bp insertion at −183 | This study |
| pRLZ12 | Same as for pRLZ1 except has 118-bp insertion at −183 | This study |
| pRLZ13 | Same as for pRLZ1 except has 232-bp insertion at −183 | This study |
a
Apr, Kmr, Tcr, and Spr denote resistance to ampicillin, kanamycin, tetracycline, and spectinomycin, respectively. LacZ−, produced no detectable β-galactosidase activity; EPS−, nonmucoid and deficient in production of exopolysaccharide; nt, nucleotide.