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. 1998 May;180(10):2736–2743. doi: 10.1128/jb.180.10.2736-2743.1998

TABLE 2.

Effect of site-directed mutations in the −315 palindromic region of the xpsR promoter on expression and regulation

Plasmid PxpsR nucleotide sequence fused to lacZ Mutation Expressiona from PxpsR in:
WT vsrD
pRLZ1 −338 to +36 None 5,280 443
pRLZ15 −338 to +36 −322 CCC→TGG 775 577
pRLZ16 −338 to +36 −319 CTAA→Δ 843 575
pRLZ17 −338 to +36 −327 AAT→GTA 4,910 709
a

Expression (transcription) from PxpsR was monitored by measuring LacZ (β-galactosidase) activity in Miller units as described by Miller (23) in overnight cultures of R. solanacearum wild type (WT) or vsrD mutants (strain AW-D5) carrying pRG970 vectors with PxpsR::lacZ fusions having the indicated mutation. Values are averages from three independent experiments.