. 1998 May;180(10):2736–2743. doi: 10.1128/jb.180.10.2736-2743.1998

TABLE 4.

Effect of upstream sequences on PhcA binding and transcription activation of P_xpsR fragments with PhcA binding site mutations

Plasmid	P_xpsR nucleotide sequence fused to lacZ	Mutation	Expression^a directed from P_xpsR in:			PhcA binding^b
Plasmid	P_xpsR nucleotide sequence fused to lacZ	Mutation	WT	vsrD	phcA	PhcA binding^b
pRLZ1	−338 to +36	None	5,737	469	86	100
pRMB	−338 to +36	−82 TAAAAA→AGATCT	1,978	138	90	50
pRM7	−338 to +36	−83 T→C	903	56	43	35
pRM7-M	−286 to +36	Same as that for pRM7	225	185	64	NT
pRMB-M	−286 to +36	Same as that for pRMB	290	214	83	NT

Expression (transcription) from P_xpsR was monitored by measuring LacZ (β-galactosidase) activity in Miller units in cultures of R. solanacearum wild type (WT), AW1-80 (phcA mutant), and AW-D5 (vsrD mutant) carrying pRG970 vectors with P_xpsR::lacZ fusions having the indicated mutation. Values are averages from three independent experiments with <20% variation.

PhcA binding was quantified as described in Table 3, footnote b, on gels like those shown in Fig. 4. NT, not tested.