Table 1.
The primer sequences for the real-time PCR
| Gene | Forward (5' > 3') | Reverse (5' > 3') |
|---|---|---|
| IRS1 | CCCAGGACCCGCATTCAAA | GGCGGTAGATACCAATCAGGT |
| PI3K p100α | CCCAGGTGGAATGAATGGCT | AGCACCCTTTCGGCCTTTAA |
| AKT | AGCGACGTGGCTATTGTGAAG | GCCATCATTCTTGAGGAGGAAGT |
| GLUT2 | GCCTGGTTCCTATGTATATCGGT | GCCACAGATCATAATTGCCCAAG |
| PEPCK | GGCTGAGAATACTGCCACACT | ACCGTCTTGCTCTCTACTCGT |
| G6Pase | ACTGGCTCAACCTCGTCTTTA | CGGAAGTGTTGCTGTAGTAGTCA |
| ATF6α | AGCAGCACCCAAGACTCAAAC | GCATAAGCGTTGGTACTGTCTGA |
| GRP78 | CATCACGCCGTCCTATGTCG | CGTCAAAGACCGTGTTCTCG |
| CHOP | TTCTCTGGCTTGGCTGACTG | TCCTCCTCTTCCTCCTGAGC |
| GAPDH | TCCAAAATCAAGTGGGGCGA | TGATGACCCTTTTGGCTCCC |
IRS1 Insulin receptor substrate 1, PI3K p100α Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, Akt Serine/threonine kinase 1, GLUT2 Glucose Transporter 2, PEPCK Phosphoenolpyruvate carboxykinase 2, G6Pase Glucose-6-phosphatase catalytic subunit 1, ATF6α Activating transcription factor 6 alpha, GRP78 G protein-coupled receptor 78, CHOP C/EBP homologous protein, GAPDH Glyceraldehyde-3-phosphate dehydrogenase