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. 2023 Dec 15;21:354. doi: 10.1186/s12964-023-01378-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Enrichment of phosphatidylinositol and PI3K-Akt pathways in the phospho-proteomes of ligand stimulated EPHB1 mutants with reduced or compromised compartmentalisation phenotypes. One mutant representing each category of compartmentalisation phenotype, C61Y (compromised), D762N (reduced), R351L (wild-type-like) and R743W (enhanced) along with wild-type EPHB1 were subjected to Ephrin-B1-Fc ligand stimulation for 30 min followed by array-based differential phospho-proteome (A) or total proteome (B) analysis. The Fc-stimulated wild-type EPHB1 served as negative control. Protein and phosphorylation levels of 1,438 different proteins were analysed using the Sciomics platform using 1,929 antibodies. Up- and down-regulated phospho-proteins and total proteins in red and blue, respectively. The enriched pathway from the STRING database (https://string-db.org) were shown on the differential phosphorylated proteins of EphrinB1-Fc ligand stimulated EPHB1 (C61Y/D762N/R351L) mutants