Figure 4. The topological position of signature mutations emerged in the 2022 outbreak in MPXV F8L.
– Panel a depicts the signature mutation W411L in the poxvirus-specific ‘insert 2’ (rendered as forestgreen ribbons). The relative position of the 3’- 5’ exonuclease site (D268) is also a reference. Panel b shows that the 2022 MPXV signature mutation L108F (ball-and-stick) within the N-terminal domain (represented as orange ribbons) stacks against the template base as seen in the cryo-EM structure of MPXV DNA replication holoenzyme (PDB entry 8HG1).