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. 2023 Dec 6;3(1):vbad179. doi: 10.1093/bioadv/vbad179

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — escheR enables multidimensional spatial visualizations following the Gestalt principles of design. (A and B) The traditional visualization displays the colocalization plot of the expression of two genes EFNA5 and EPHA5 (A) and the spatial domains from the dorsolateral prefrontal cortex in postmortem human brain (Huuki-Myers et al. 2023) (B) side-by-side, creating challenges to cognitively connecting colocalization status to spatial domains. (C) The watercolor effect enables displaying spatial domains by color-coding only outlines of circles. (D) escheR enables the multidimensional in situ visualization that simultaneously displays the cortex layers and the colocalization status, substantially improving interpretability.