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Western blot showing time course study of O-GlcNAcylation response to nutrient deprivation in HeLa cells stably expressing OGT (W) and OGT-N791A (M). Cells were induced for 48 hours prior to high or low nutrient treatment, then samples were collected at 3, 8, and 24 hours of treatment. Western blots are representative of three biological replicates.
