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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Nat Chem Biol. 2023 Aug 31;19(11):1423–1431. doi: 10.1038/s41589-023-01422-2

Figure 3. Int-D binding site is a regulator of protein association and O-GlcNAcylation.

Figure 3.

(a) Co-IP of cMyc-SMG9 with Flag-OGT (W) or Flag-OGT-N791A (M) from TRex-293 cells, followed by western blot detection. (b) Co-IP of cMyc-SMG9 (W) or cMyc-SMG9-Y147F (M) with Flag-OGT from TRex-293 cells, followed by western blot detection. (c) O-GlcNAcylation detection on SMG9 (W) or SMG9-Y147F (M) from cells co-expressed with Flag-OGT (W) or Flag-OGT-N791A (M). cMyc-tagged SMG9 (WT or Y147F mutant) was immunoprecipitated from TRex-293 cell lysate by cMyc-agarose, biotinylated by GalT assay, and detected by streptavidin-HRP far western blot. All whole cell lysates have endogenous OGT knocked down. Blots are representative of at least three biological replicates.