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Expression kinetics after eGFP-mRNA LNP transfection in vitro. (A) Exemplary single-cell fluorescence time courses of mRNA-Cy5 and eGFP (B) Correlation of transfection efficiency and onset time of single cells reveals delayed and less efficient expression for DD vs. MC3 and KC2. (C) eGFP-onset typically occurs 0.9 h after the event of Cy5-signal increase.
