Figure 3.

Representative detection of sCJD prions from patient urine samples. The figure shows the results of western blots from samples amplified by three rounds of PMCA starting with urine from different sCJD patients and controls (both healthy individuals and patients affected by other neurological diseases). Each number represents a urine sample from a different patient. For most sCJD subtypes, results from 10 samples are shown, except for MV1 and VV1 where we were able to obtain only eight and three patient samples, respectively. All MM2 samples analyzed in this study were MM2C. Prior to PMCA, urine samples were processed by IOME extraction as described in methods. To further evaluate the sensitivity of PMCA detection, urine samples were evaluated in both 129M and 129V substrates. The dotted line indicates blot splicing to remove nonrelevant lines.