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. 1998 May;180(10):2766–2769. doi: 10.1128/jb.180.10.2766-2769.1998

FIG. 2.

FIG. 2

Primer extension analysis of algD transcription in strains SMU88 and ATCC 9046, with and without plasmid pSMU865. (A) DNA sequence of the 5′ end of algD. The arrows indicate the start sites of algD transcription, the p1 and p2 promoters are indicated (overbar), and the complementary sequences where oligonucleotides algD1 and algD2 (used for primer extension analysis) were generated are underlined. The algD ATG initiation codon is shown in boldface type. (B and C) Primer extension of the algD gene with oligonucleotide algD1 in strains SMU88 (lane 1) and ATCC 9046 with (lane 2) and without (lane 3) plasmid pSMU865 (B) and with oligonucleotide algD2 in strain SMU88 (C). The algD sequence ladders (GATC) were produced with the oligonucleotides used for primer extension.