Extended Data Figure 1.
(a) Viability levels of human PDAC cell lines with KRASG12X (AsPC-1, HPAC, Panc 05.04, PANC-1, Panc 10.05, PL45, SU.86.86, SW-1990, KP-4, HPAF-II, Capan-1, Capan-2, CFPAC-1, Panc 03.27, HuP-T4, MIA PaCa-2 and PSN-1), KRASQ61H (Hs 766T), and BRAFΔV487-P492 (BxPC-3) mutations treated with indicated concentrations of RMC-7977 for 4 hours. Data points represent the mean of technical replicates normalized to DMSO control. Error bars indicate s.d. KRAS mutations are indicated by curve colors. (b) Representative Western Blot images for three murine PDAC cell lines treated with DMSO or range of RMC-7977 concentrations (1–100 nM) for 2 hours. Protein levels of phospho-ERKT202/204 and total ERK were analyzed. Alpha-(α)-tubulin was used as loading control. (c) Representative Western Blot images for human PDAC cell lines treated with DMSO or range of RMC-7977 concentrations (1–100 nM) for 24 hours. Protein levels of phospho-ERKT202/204, total ERK, phospho-pS6S235/236 total S6, phospho-AktS473 and total Akt were analyzed. Vinculin was used as loading control. (d) Representative Western Blot images for AsPC-1 cell line treated with DMSO or RMC-7977 (100 nM) for indicated timepoints. Protein levels of phospho-ERKT202/204, total ERK, total PARP and cleaved PARP were analyzed. Vinculin was used as loading control.