Fig. 2: WS-dependent copulation success and song modulation in D. santomea.

a, Proportion of pairs with intact or wing-cut (WC) females that succeeded in copulation in each species. Error bars represent the 95% confidence interval. Fractions at the base of each bar denote “number of pairs that copulated”/“total pairs tested”. Significance tested by Fisher’s exact test.

b, Mean length of pulse trains separated by whether they elicited WS and whether the pair copulated during the recording period. Dot size corresponds to the number of pulse trains of each type in each pair.

c,d, Mean latency of WS from pulse train onset (c) and mean pulse train length after WS onset (d), respectively, separated by whether the pair copulated during the recording period. In (d), the non-parametric Mann-Whitney U test is used to test for statistical difference between the two groups.

e, Mean length of pulse trains in pairs with intact females, separated by whether they elicited WS, and in pairs with WC females. Only pairs that did not copulate during the recording period are shown.

Dot size in b-e corresponds to the number of pulse trains of each type in each pair. Unless otherwise specified, error bars show mean±SEM and statistical significance was tested with linear models.. *** p<0.001, * 0.01<p<0.05, n.s. not significant.