Skip to main content
[Preprint]. 2023 Dec 6:2023.12.05.570116. [Version 1] doi: 10.1101/2023.12.05.570116

Fig. 5. Glutamate release is faster in aggregating C. elegans, independent of NRX-1.

Fig. 5.

A) Cartoon of sra-6p∷eat-4∷pHluorin experiment, including schematic of small neuron section bleached and EAT-4∷pHluorin photobleaching and recovery process. B) Representative images of ASH neuron prior to bleaching (pre-bleach), during bleach, immediately following bleach, and after recovery period of two minutes (Scale bar = 5μm). C) Graph showing initial fluorescence values taken from first 10 pre-bleach frames of FRAP experiments. D) Graph of post-bleach recovery as a fraction of initial fluorescence by post-bleach frame up to frame 138 (120 seconds, frame taken every 0.87 seconds) Comparisons shown on graph include: npr-1 and npr-1;nrx-1 (p=0.278), solitary control and nrx-1(p=0.080), solitary control and npr-1 (dark blue, p<0.0001), and npr-1;nrx-1 and nrx-1 (light blue, p<0.0001).