Figure 5. Large-scale EM reveals novel cilia disassembly intermediates in differentiating GCs.

(A, B, and D) Serial sections of representative short, concealed cilia (A), protein-rich short cilia (B) and cilia with internal vesicles (D) are displayed. The section orientation is indicated by the cartoon on the left and the 3D reconstructions of each cilium is displayed on the far right.

(C) Mother centriole depth is plotted on an inverted y-axis such that higher depth values fall farther below the Y-axis. To the left of the dotted vertical line, the centrioles are classified by layer, with the outer EGL the cells broken out by cell cycle phase. On the right side of the dashed line, the mother centrioles are grouped based on the type of cilia deconstruction intermediates observed.

(E) Two representative EM sections showing invagination of the ciliary membrane are shown to the left of the 3D reconstruction of the concealed cilium from the EGL.

(F – L) For each centriole, two representative EM sections are displayed to the left of the 3D reconstruction. The classification of the centriole is indicated as ciliary vesicle (F-H), docked centriole (I-J), tethered centriole (K), or cytoplasmic centriole (L). On the EM images, yellow transparent overlay indicates ciliary vesicles, blue highlights the cell boundary and purple encircles the centriole.

(K) The percentage of GCs in the IGL with centrioles in each classification are quantified. All scale bars are 200 nm.