Figure 2. Gsx proteins bind a consensus DNA binding motif with high affinity.

Isotherms from isothermal titration calorimetry (ITC) depict the binding activity of Gsx2^HD, Gsx1^HD, and Gsx2^167-305 to both a -TAATTA- and -TAATGG- motif. (A) The Gsx2^HD shows an average 15 nM affinity for the -TAATTA- site and a 36 nM affinity for the -TAATGG- site. (B) The Gsx1^HD shows nearly identical affinities to Gsx2^HD, with an average 18 nM and 42 nM affinity for the -TAATTA- and -TAATGG- sites, respectively. (C) Conversely, the Gsx2^167-305 protein containing short N- and C-terminal flanking regions shows weaker affinity with 77 nM and 143 nM affinity for the -TAATTA- and -TAATGG- sites, respectively.