Table 1.

Calorimetric data of Gsx2 and Gsx1 HD constructs binding to DNA monomer sites

Cell (Protein 10uM)	Syringe (DNA 100uM)	Temp.	N	K	Kd	ΔG○	ΔH○	−TΔS○
		○C		M−1 (x107)	nM	kcal/mol	kcal/mol	kcal/mol
Gsx2 203–264	TGAGCTAATGGAAGC	20	0.98	2.8 ± 1.4	36	−9.9 ± 0.4	−6.6 ± 0.4	−3.3 ± 0.8
Gsx1 146–207	TGAGCTAATGGAAGC	20	0.97	2.4 ± 0.9	42	−9.9 ± 0.2	−5.5 ± 0.1	−4.4 ± 0.2
Gsx2 167-305	TGAGCTAATGGAAGC	20	0.94	0.7 ± 0.2	143	−9.2 ± 0.2	−8.3 ± 0.4	−0.9 ± 0.5
Gsx2 203–264	TGAGCTAATTAAAGC	20	1.02	6.8 ± 4.2	15	−10.4 ± 0.5	−5.4 ± 0.5	−5.0 ± 0.9
Gsx1 146–207	TGAGCTAATTAAAGC	20	0.96	5.5 ± 0.8	18	−10.4 ± 0.1	−5.2 ± 0.3	−5.2 ± 0.2
Gsx2 167-305	TGAGCTAATTAAAGC	20	0.85	1.3 ± 0.6	77	−9.5 ± 0.2	−8.9 ± 0.1	−0.6 ± 0.3
Gsx2I234E 203–264	TGAGCTAATTAAAGC	20	1.05	5.1 ± 4.2	20	−10.2 ± 0.5	−5.2 ± 0.5	−5.0 ± 0.9

^*Constructs Gsx2 203-264 and Gsx1 146-207 correspond to their respective homeodomains, while Gsx2 167-305 includes the homeodomain plus flanking regions N- and C-terminal of the homeodomain. Values are the mean of at least three independent experiments, and the errors represent the standard deviation.