Figure 1: Schema of the experimental workflow applied in this study to explore transcriptome changes upon DHA exposure.

This schematic diagram illustrates the study design, points of sample collection and treatment, sample processing and single cell library preparation using the SeqWell platform. Both the wildtype (K13^C580 strain) and mutant (K13^580Y mutant strain) Pf parasite cell lines were cultured separately for 48hrs. The control (untreated) parasite was collected for library prep. The controls for each parasite strain were then divided into two groups, some treated with 700 nM DHA (lethal dose of DHA) and DMSO. After DHA and DMSO treatment or exposure, samples were collected after every 2 hrs for single cell library preparation using SeqWell.