Table 2.
Plasmids used to generate pseudotyped viruses.
| Viral glycoprotein | Accession | Vector | Target cells |
|---|---|---|---|
| Coronaviridae | |||
| SARS-CoV S (Tor2) | NC_004718.3 | pcDNA 3.1+ | HEK293T ACE2 + TMPRSS2 |
| SARS-CoV-2 S (Wuhan) | NC_045512.2 | pcDNA 3.1+ | HEK293T ACE2 + TMPRSS2 |
| RaTG13 S | QHR63300.2 | pcDNA 3.1+ | HEK293T ACE2 + TMPRSS2 |
| Filoviridae | |||
| EBOV Mayinga 76 GP | EU224440 | pCAGGS | CHO |
| MARV Angola 05 GP | DQ447660 | pCAGGS | CHO |
| Orthomyxoviridae | |||
| IAV-like H17N10 H17 | AFC35438.1 | pI.18 | MDCK II |
| Paramyxoviridae | |||
| NiVb G and F | JN808864.1 | pCAGGS | BHK |
| GH-M74A G and F | HQ660129 | pCAGGS | BHK |
HEK293T cells were transfected with Angiotensin-converting enzyme 2 (ACE2) and Transmembrane protease, serine 2 (TMPRSS2). CHO, Chinese hamster ovary cells; MDCK, Madin-Darby canine kidney cells. For this study we used the G and F genes from a Bangladeshi NiV isolate (NiVb).