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The menA plasmids. pMK2 was constructed by insertion into pUC18 of a 2.0-kb PstI fragment derived from pLC28-47; pMK3 has an EcoRV fragment deletion of pMK2; pMK4 contains only the EcoRV fragment of pMK2; and pMK5 and pMK6 contain the 0.5- and 1.5-kb HindIII/PstI fragments of pMK2, respectively. Complementation was assayed by anaerobic growth on glycerol medium with TMAO as the electron acceptor. B, BglII; D, HindIII; E, EcoRI; H, HincII; P, PstI; V, EcoRV.
