TABLE 1.
Strains of E. coli K-12 and plasmids used in the study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| E. coli | ||
| PL2024 | gal trpA trpR iclR rpsL | 17, 20 |
| AN67 | Δ(gpt-proA)62 lacY1 tsx-29 supE44 galK2 λ− trpE81 pheA3::Mu tyrA4 xyl-5 mtl-1 menA401 thi-1 | 21 |
| HB101 | supE44 hsdS20 (rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Laboratory stock |
| JM83 | F−ara Δ(lac-proAB) rpsL (Strr) [φ80Δ(lacZ)M15] | Laboratory stock |
| Plasmid | ||
| pUC18 | Cloning vector | International Biotechnologies, Inc. |
| pUC19 | Cloning vector | International Biotechnologies, Inc. |
| pLC28-47 | Clarke-Carbon plasmid clone | 16 |